Hello, I'm new in the forum and I hope someone can help out with my problem.
We have run a trial during which we assessed changes in flesh colour (a score was assigned) in two areas of the fillet over time.
At each time point A, B, C, D, we sampled fish from the same cage. During A we could sample only 30 fish while we sampled 50 in all the following time points. As you imagine the fish sampled and measured where different individuals at each time point as we had to sacrifice them to check the flesh colour.
What I want to do is:
-comparing colour (for each of the two areas assessed independently) between time point and obtain a kind of pair-wise comparison such as AvsB,C,D,BvsC,D,CvsD to see between which time points the difference is significant.
-comparing colour between the two areas of the fillet assessed (which tend to lose colour at a different rate) at each time point
Which kind of analyses should I use? and how should I organise the data?
Please help me!!!