"Males" and "females" aren't in two columns, they are just in one column. Here is an example: In one of my studies, my student created a hypothetical applicant scenario. In one scenario, the applicant was introverted and in the other scenario, the applicant was extraverted. So, I conducted an independent samples t test to see if there were differences between these groups. There are statistically significant differences between the groups. However, I'd like to know if the females had different preferences for a particular applicant than did males. I'm not sure how to do this.